caption a7 strain plasmid relevant genotype  (ATCC)

Journal:

Article Title: Bartonella bacilliformis GroEL; Effect on Growth of Human Vascular Endothelial Cells in Infected Co-Cultures

doi: 10.1196/annals.1355.046

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: HUVEC use was restricted to tissue culture passages 1–4 to minimize variability between experiments. table ft1 table-wrap mode="anchored" t5 caption a7 Strain/Plasmid Relevant Genotype/Description Source/Reference B. bacilliformis KC583 Neotype strain ATCC ( 3 ) JB584 Transformation-competent strain ( 2 ) LSS100 JB584 containing pGRO1 ( 16 ) LSS001 JB584 containing pBBR1-MCS2 ( 16 ) LSS200 JB584 containing pGRO1Δ This study E. coli DH5α Host for plasmid propagation Gibco-BRL, Grand Island, N.Y. Plasmids pBBR1MCS-2 B. bacilliformis shuttle vector, Km r ( 2 , 14 ) pGRO1 pBBR1MCS-2 with cloned B. bacilliformis groESL operon ( 16 ) pGRO1Δ pGRO1 with 1002-bp deletion in groEL and four intervening XhoI linkers This study Open in a separate window Bacterial strains and plasmids used in this study B. bacilliformis - HUVEC tissue co-cultures The effect of B. bacilliformis infection and over-expression of GroEL on HUVEC growth and morphology was examined in co-cultures.

Techniques: Transformation Assay, Plasmid Preparation, Clone Assay

Journal:

Article Title: Bartonella bacilliformis GroEL; Effect on Growth of Human Vascular Endothelial Cells in Infected Co-Cultures

doi: 10.1196/annals.1355.046

Figure Lengend Snippet: Dose-response of HUVECs cultured 96 h in EGM supplemented with a range of B. bacilliformis KC583 cell lysate concentrations as indicated, showing mitogenic activity at 1–10 μg/ml and inhibition of growth at 100–300 μg/ml dosages. Data represent the average of three experiments ± SD. (Raw, raw lysate; heated, lysate pretreated at 100° C for 7 min).

Article Snippet: HUVEC use was restricted to tissue culture passages 1–4 to minimize variability between experiments. table ft1 table-wrap mode="anchored" t5 caption a7 Strain/Plasmid Relevant Genotype/Description Source/Reference B. bacilliformis KC583 Neotype strain ATCC ( 3 ) JB584 Transformation-competent strain ( 2 ) LSS100 JB584 containing pGRO1 ( 16 ) LSS001 JB584 containing pBBR1-MCS2 ( 16 ) LSS200 JB584 containing pGRO1Δ This study E. coli DH5α Host for plasmid propagation Gibco-BRL, Grand Island, N.Y. Plasmids pBBR1MCS-2 B. bacilliformis shuttle vector, Km r ( 2 , 14 ) pGRO1 pBBR1MCS-2 with cloned B. bacilliformis groESL operon ( 16 ) pGRO1Δ pGRO1 with 1002-bp deletion in groEL and four intervening XhoI linkers This study Open in a separate window Bacterial strains and plasmids used in this study B. bacilliformis - HUVEC tissue co-cultures The effect of B. bacilliformis infection and over-expression of GroEL on HUVEC growth and morphology was examined in co-cultures.

Techniques: Cell Culture, Activity Assay, Inhibition